No. The $35 application fee is a one-time purchase that allows you to use your application for any participating rentals for 30 days. If you have not secured housing at the end of the 30 day period, and wish to repurchase the application, you may do so.
Please note that the charge can appear as recurring, or a subscription on some bank statements because it is a 30-day service, however, it is a one-time purchase and you will not be auto-charged at the end of the 30-day time period.